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kapa htp library preparation kit  (Roche)


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    Structured Review

    Roche kapa htp library preparation kit
    Kapa Htp Library Preparation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kapa+htp+library+preparation+kit/KAPA+RNA+Kit/pm41064934-44-7-12
    Average 99 stars, based on 1 article reviews
    kapa htp library preparation kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Construct:

    Article Title: Genomic and Transcriptional Profiling of Chinese Melanoma Patients Enhanced Potentially Druggable Targets: A Multicenter Study
    Article Snippet: DNA fragments of 200~400 bp were purified by an Agencourt AMPure XP Kit (Beckman Coulter, CA, USA). .. Fragmented genomic DNA libraries were constructed with the KAPA HTP Library Preparation Kit (Kapa Biosystems, Wilmington, MA, USA) according to the instruction manual. ..

    Article Title: Genomic and Transcriptional Profiling of Chinese Melanoma Patients Enhanced Potentially Druggable Targets: A Multicenter Study
    Article Snippet: Qualified RNA was used to synthesize double-stranded cDNA using a SuperScript VILO cDNA Synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA) and an Abclonal Second Strand Synthesis Module (Abclonal, Wuhan, China) according to the recommended protocol. .. To prepare genomic libraries, the double-stranded cDNA was fragmented and constructed by a KAPA HTP Library Preparation Kit (KAPA Biosystems, Wilmington, MA, USA). .. The prepared libraries were captured with FusionCaptureTM (Genetron Health, Beijing, China), which contains 395 cancer-related genes, and then subjected to Illumina NovaSeq6000 (Illumina, Inc., San Diego, CA, USA) for paired-end sequencing.

    Amplification:

    Article Title: Identification of MET fusions in solid tumors: A multicenter, large scale study in China.
    Article Snippet: Department of Digestive Oncology, Cancer Center, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, Shanxi, China Jichenjunchuang Clinical Laboratory, Hangzhou, China Department of Gynecologic Oncology, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, China Key Laboratory of Gastroenterology of Zhejiang Province, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China Zhejiang Provincial People's Hospital, Hangzhou, China Department of Neurosurgery, Xiangya Hospital Central South University, Changsha, China

    Adapter Ligation:

    Article Title: Systematic perturbation screens decode regulators of inflammatory macrophage states and identify a role for TNF mRNA m6A modification
    Article Snippet: From the estimated 3 to 6 million cells in each of these bins, 20 to 40 % of genomic DNA was recovered by mass. sgRNA guide sequences were recovered as amplicons generated by PCR of the genomic DNA using Phusion High-Fidelity PCR Master Mix with GC Buffer (M0531L, NEB) with forward (TCTTGTGGAAAGGACGAGGTACCG) and reverse (TCTACTATTCTTTCCCCTGCACTGT) primers representing complimentary guide flanking sequences. .. Adapter ligation and indexing PCR was performed on these amplicons using KAPA HTP Library Preparation Kit (KK8234, Kapa biosystems) for multiplexing and sequencing on an Illumina HiSeq5 platform. .. Staining proceeded with an initial Fc-block step (BD553142, BD bioscience) combined with fixable viability dye eFluor 780 (65-0865-14, eBioscience).

    Polymerase Chain Reaction:

    Article Title: Systematic perturbation screens decode regulators of inflammatory macrophage states and identify a role for TNF mRNA m6A modification
    Article Snippet: From the estimated 3 to 6 million cells in each of these bins, 20 to 40 % of genomic DNA was recovered by mass. sgRNA guide sequences were recovered as amplicons generated by PCR of the genomic DNA using Phusion High-Fidelity PCR Master Mix with GC Buffer (M0531L, NEB) with forward (TCTTGTGGAAAGGACGAGGTACCG) and reverse (TCTACTATTCTTTCCCCTGCACTGT) primers representing complimentary guide flanking sequences. .. Adapter ligation and indexing PCR was performed on these amplicons using KAPA HTP Library Preparation Kit (KK8234, Kapa biosystems) for multiplexing and sequencing on an Illumina HiSeq5 platform. .. Staining proceeded with an initial Fc-block step (BD553142, BD bioscience) combined with fixable viability dye eFluor 780 (65-0865-14, eBioscience).

    Multiplexing:

    Article Title: Systematic perturbation screens decode regulators of inflammatory macrophage states and identify a role for TNF mRNA m6A modification
    Article Snippet: From the estimated 3 to 6 million cells in each of these bins, 20 to 40 % of genomic DNA was recovered by mass. sgRNA guide sequences were recovered as amplicons generated by PCR of the genomic DNA using Phusion High-Fidelity PCR Master Mix with GC Buffer (M0531L, NEB) with forward (TCTTGTGGAAAGGACGAGGTACCG) and reverse (TCTACTATTCTTTCCCCTGCACTGT) primers representing complimentary guide flanking sequences. .. Adapter ligation and indexing PCR was performed on these amplicons using KAPA HTP Library Preparation Kit (KK8234, Kapa biosystems) for multiplexing and sequencing on an Illumina HiSeq5 platform. .. Staining proceeded with an initial Fc-block step (BD553142, BD bioscience) combined with fixable viability dye eFluor 780 (65-0865-14, eBioscience).

    Sequencing:

    Article Title: Systematic perturbation screens decode regulators of inflammatory macrophage states and identify a role for TNF mRNA m6A modification
    Article Snippet: From the estimated 3 to 6 million cells in each of these bins, 20 to 40 % of genomic DNA was recovered by mass. sgRNA guide sequences were recovered as amplicons generated by PCR of the genomic DNA using Phusion High-Fidelity PCR Master Mix with GC Buffer (M0531L, NEB) with forward (TCTTGTGGAAAGGACGAGGTACCG) and reverse (TCTACTATTCTTTCCCCTGCACTGT) primers representing complimentary guide flanking sequences. .. Adapter ligation and indexing PCR was performed on these amplicons using KAPA HTP Library Preparation Kit (KK8234, Kapa biosystems) for multiplexing and sequencing on an Illumina HiSeq5 platform. .. Staining proceeded with an initial Fc-block step (BD553142, BD bioscience) combined with fixable viability dye eFluor 780 (65-0865-14, eBioscience).

    Article Title: Molecular profiling in paediatric hepatocellular adenomas: phenotypic correlations and clinical significance.
    Article Snippet: Yan Zhou, Antonio R Perez-Atayde, Xuchen Zhang, Allison F O’Neill, Alanna J Church & Juan Putra Department of Pathology, Boston Children’s Hospital/Harvard Medical School, Boston, Massachusetts, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut and Dana-Farber Cancer Institute/Boston Children’s Cancer and Blood Disorders Center and Harvard Medical School, Department of Pediatric Oncology, Boston, Massachusetts, USA

    Next-Generation Sequencing:

    Article Title: Somatic RIT1 delins in arteriovenous malformations hyperactivate RAS-MAPK signaling amenable to MEK inhibition.
    Article Snippet: Indexed sequencing libraries were prepared from 50-ng sonically sheared DNA samples using Illumina TruSeq LT reagents (Illumina). .. Extracted DNA underwent targeted NGS using the KAPA HTP Library Preparation Kit (Roche), a custom RNA bait set (Agilent SureSelect) and sequenced with the Illumina HiSeq 2500 system. ..

    Article Title: Molecular profiling in paediatric hepatocellular adenomas: phenotypic correlations and clinical significance.
    Article Snippet: Yan Zhou, Antonio R Perez-Atayde, Xuchen Zhang, Allison F O’Neill, Alanna J Church & Juan Putra Department of Pathology, Boston Children’s Hospital/Harvard Medical School, Boston, Massachusetts, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut and Dana-Farber Cancer Institute/Boston Children’s Cancer and Blood Disorders Center and Harvard Medical School, Department of Pediatric Oncology, Boston, Massachusetts, USA



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